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High-Throughput Next Generation Sequencing: Methods and Applications (Methods in Molecular Biology)

Steven C. Ricke, Young Min Kwon | Humana Press | 2011-03-27 | 319 pages | English | PDF

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.

Next-Generation Genome Sequencing Book

From the first genomic landmark of deciphering the phiX174 bacteriophage genome achieved by F. Sanger’s group in 1977 (just over a 5000 bases of contiguous DNA) to sequencing several bacterial megabase-sized genomes in the early 1990s by The Institute for Genomic Research (TIGR) team, from publishing by the European Consortium the first eukaryotic genome of budding yeast Saccharomyces cerevisiae in 1996 to producing several nearly finished gigabase-sized mammal genomes including our own, Sanger sequencing definitely has come a long and productive way in the past three decades. Sequencing technology has dramatically changed the face of modern biology, providing precise tools for the characterization of biological systems. The field has rapidly moved forward now with the ability to combine phenotypic data with computed DNA sequence and therefore unambiguously link even tiny DNA changes (e.g., single-nucleotide polymorphisms (SNPs)) to biological phenotypes. This allows the development of practical ways for monitoring fundamental life processes driven by nucleic acids in objects that vary from single cells to the most sophisticated multicellular organisms.

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